ALEX BOTSARIS PDF

Z-Ligustilide LIG , the primary lipophilic component of the Chinese traditional medicine Radix Angelica Sinensis, has been shown to reduce ischemic brain injury via anti-apoptotic pathways. Mortality, neurobehavioral evaluation, brain water content, blood brain barrier BBB permeability, and vasospasm assessment of the basilar artery were measured on days 3 and 7 after injury. The results showed that LIG post-treatment reduced mortality, neurobehavioral deficits, brain edema, BBB permeability, and cerebral vasospasm. Additionally, treatment reduced the number of apoptotic cells in the surrounding brain injury site which accompanied a marked down-regulation of pro-apoptotic proteins, p53 and cleaved caspase

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Z-Ligustilide LIG , the primary lipophilic component of the Chinese traditional medicine Radix Angelica Sinensis, has been shown to reduce ischemic brain injury via anti-apoptotic pathways. Mortality, neurobehavioral evaluation, brain water content, blood brain barrier BBB permeability, and vasospasm assessment of the basilar artery were measured on days 3 and 7 after injury.

The results showed that LIG post-treatment reduced mortality, neurobehavioral deficits, brain edema, BBB permeability, and cerebral vasospasm. Additionally, treatment reduced the number of apoptotic cells in the surrounding brain injury site which accompanied a marked down-regulation of pro-apoptotic proteins, p53 and cleaved caspase In the anticoagulative assay, compounds 1 and 2 exhibited weak antithrombin activity and strong antiplatelet aggregation activity in vitro.

Diels that has been used in traditional Chinese medicine for treating gynecological disorders and anemia. The present study aims to clarify the inhibitory effect of ASP on hepcidin expression as well as the involved mechanisms. Erythropoietin and hepcidin levels in serum at different time points were determined by enzyme-linked immunosorbent assay. Western blot was used to investigate the expression of 6 pertinent signal proteins in liver. The morphological changes of kidneys were also semi-quantitatively analyzed by HE, Masson stained tissue sections, which including interstitial cell infiltration, tubular atrophy and interstitial fibrosis.

However, the following 4 parts extracted from IC in the third extraction were shown no effect on the above indexes. As a step further to identify and characterize the active chemical components of DBT, we tested the hematopoietic and particularly, thrombopoietic effects of polysaccharide-enriched fractions from the root of Radix Angelicae Sinensis APS in this study. Peripheral blood cells from APS, thrombopoietin and vehicle-treated samples were then counted at different time-points. Using a megakaryocytic cell line Me as model, we analyzed the cellular apoptosis progression with and without APS treatment by Annexin V, Mitochondrial Membrane Potential and Caspase 3 assays.

In Me cells, we observed the anti-apoptotic effect of APS. Treatment by Ly alone increased the percentage of cells undergoing apoptosis. However, addition of APS to Lytreated cells significantly reduced the percentage of cells undergoing apoptosis.

Angelica sinensis modulates immunohematopoietic response and increases survival of mice infected with Listeria monocytogenes. The effects of a dry extract of the roots of Angelica sinensis Oliv. Extraction, chemical analysis of Angelica sinensis polysaccharides and antioxidant activity of the polysaccharides in ischemia-reperfusion rats.

The major sugar of the polysaccharide was saccharose Glucose and fructose were found as minor components of the polysaccharides. Absorptions at cm -1 are attributed to asymmetrical stretching vibration of CH 2 -group.

Medium stretch observed in the range cm -1 is assigned to C-C stretching of polysaccharides. Cardioprotective effects of A. The study strongly suggests the cardioprotective activity of A.

Hematopoietic effect of water-soluble polysaccharides from Angelica sinensis on mice with acute blood loss. The AS, ASPS, and small molecular weight compounds were incubated with mice spleen cells to obtain conditioned mediums, and then their hematopoietic activities were evaluated by granulocyte macrophage GM colony-forming assay in vitro.

During in vivo test, we used mice that were bled approximately 0. The hematopoietic activity was through the stimulation of secretion of interleukin-6 and GM colony-stimulating factor, and the amounts of these hematopoietic growth factors secreted, in general, agreed with the number of GM colony formations.

Administration of low-dose ASPS 2. Z-ligustilide extracted from Radix Angelica Sinensis decreased platelet aggregation induced by ADP ex vivo and arterio-venous shunt thrombosis in vivo in rats. Antithrombotic therapy has become an important goal for the treatment of ischemic disorders such as cerebral ischemia. Our recent studies found that Z-ligustilide LIG , a characterized 3-n-alkylphthalide constituent of Radix Angelica sinensis essential oil, exerted significant neuroprotection against cerebral ischemic damage in several animal models.

The present study evaluated the antithrombotic activity of LIG and its effect on platelet aggregation and coagulation time. Our results showed that LIG significantly and dose-dependently reduced arterial thrombus weight in an arteriovenous shunt thrombosis in rats and platelet aggregation induced by adenosine diphosphate in rats ex vivo.

The present study demonstrated for the first time that LIG may exert efficient antithrombotic activity through inhibition of platelet aggregation, without effecting coagulation time of peripheral blood. These data, together with the previously reported neuroprotective effects of LIG on cerebral ischemia, suggest that the antithrombotic activity of LIG may contribute to its potential for the treatment of ischemic diseases, including ischemic stroke.

It has been known that the renal microvasular lesions could aggravate the progress of glomerular sclerosis and tubulo-interstitial fibrosis in chronic kidney diseases.

Modern pharmacological studies indicated that the two traditional Chinese herbs, Astragalus membranaceus and Angelica sinensis, could improve micorvascular lesions through multiple mechanisms, including increasing local renal blood flow to lessen the hypoxic renal injury, promoting the recovery of renal blood flow and glomerular filtration rate after ischemia-reperfusion, modulating the imbalance of vaso-activators such as nitric oxide and angiotensin, increasing the expression of vascular epithelial growth factor and inhibiting the release of the intracellular calcium ion and promoting DNA synthesis in endothelial cells to improve the function of endothelial cells.

These evidences suggest that Astragalus membranaceus and Angelica sinensis may retard the progress of renal diseases through the above-mentioned mechanisms. Angelica sinensis and its alkylphthalides induce the detoxification enzyme NAD P H: quinone oxidoreductase 1 by alkylating Keap1. The roots of Angelica sinensis Oliv. Diels Dang Gui; Apiaceae have a long history in traditional Chinese medicine as a remedy for women's disorders and are often called "lady's ginseng".

Currently, extracts of A. In the present study, we examined the potential chemopreventive activity of A. The lipophilic partitions showed strong NQO1 induction with concentrations to double the enzyme activity CD of 5. Transcription of many detoxifying enzymes is regulated through the antioxidant response element ARE and its transcription factor Nrf2, which is repressed under basal conditions by Keap1.

However, exposure to electrophilic inducers that alkylate Keap1 results in higher concentrations of free Nrf2 and ARE activation. Under these conditions, both the extract and the ligustilide increased ARE-luciferase reporter activity in a dose-dependent manner. These observations suggest that A. Study of the anti-proliferative effects and synergy of phthalides from Angelica sinensis on colon cancer cells.

Angelica sinensis is a Chinese medicinal herb for treating gynecological and gastrointestinal disorders, and also in conjunction with cancer chemotherapy. Moreover, the results obtained in both human colon cancer HT and normal colon CCDCo cells were compared for the investigation of selectivity. Interactions among phthalides were determined by median-effect analysis. Angelica sinensis extract demonstrated significant synergy in inhibiting cell proliferation.

Angelica and ChuanXiong are used to cure ischemic heart disease in China. Previous studies found that these two herbs could increase myocardial blood flow, oxygen-supply and keep myocardial oxygen balance, etc.

However, the mechanisms of angiogenic effects of these two herbs are not well-known. The purpose of this study was to assess the effects of Angelica and ChuanXiong on vascular endothelial growth factor VEGF expression in rat myocardial infarction, on endothelial cell proliferation and quantity of vessels on chick embryo chorioallantoic membrane CAM.

In this study, rats were divided randomly into either pre-treatment or acute-treatment group and sacrificed at the end of the treatments. Similar results were observed in immunohistochemistry of VEGF expression in the myocardia.

The data showed that Angelica and ChuanXiong could affect VEGF expression in rat myocardial infarction, promote endothelial cell proliferation and stimulate quantity of vessels on CAM model. The results suggest that Angelica and ChuanXiong have angiogenic effects, and may provide some mechanisms for the treatment of myocardial infarction and peripheral ischemia. A Chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis induces the expression of erythropoietin in cultured Hep3B cells.

According to Chinese medicinal theory, this decoction is to nourish the blood function; this, however, has not been demonstrated on the molecular level. In order to reveal the hematopoietic effect of this decoction, DBT was applied to cultured Hep3B human hepatocellular carcinoma cells.

The current results verified the hematopoietic function of this ancient herbal decoction. Protective effects of Angelica sinensis extract on amyloid beta-peptide-induced neurotoxicity. The protective effects of alcohol extract from the root of Angelica sinensis AS on beta-amyloid peptide Abeta -induced toxicity and the mechanism of these effects were investigated. Abeta is a pathological hallmark of Alzheimer's disease; it decreased viability of Neuro 2A cells in a concentration-dependent manner with IC 50 of The Abeta-treated cells also showed a significant decline in the mitochondrial transmembrane potential DeltaPsim and increase in the mitochondrial volume, and portions of the cytoplasm were sequestered by a membrane-bound vacuole.

The results of this study demonstrated that AS extract possessed the activity to prevent the neurotoxicity induced by Abeta-associated oxidative stress, implying that AS has a potential role in the prevention of Alzheimer's diseases. Effect of Angelica sinensis polysaccharide-iron complex on iron deficiency anemia in rats. There was a significant difference among the changes of the indices in all the APIC groups and those of the model group but not between those of the APIC groups and the positive control group.

However, the recovery of the indices in the APIC groups was superior to that in the positive control group. Hence, it may be used as a new iron-supplementing agent with a double therapeutic efficacy on blood supplementation for the treatment of IDA. This study was designed to identify and characterize the mechanism of macrophage activation by AAP, an acidic polysaccharide fraction isolated from the roots of Angelica sinensis Oliv.

As a result, AAP significantly enhanced nitric oxide NO production and cellular lysosomal enzyme activity in murine peritoneal macrophages in vitro and in vivo. Angelica sinensis. J Pharmacol Exp Ther. J Ethnopharmacol. Zhongguo Zhong Yao Za Zhi. J Med Food. Epub Sep Int J Biol Macromol. Epub Aug 5. Exp Hematol. Epub Mar Yakugaku Zasshi. Abstract Antithrombotic therapy has become an important goal for the treatment of ischemic disorders such as cerebral ischemia.

Abstract It has been known that the renal microvasular lesions could aggravate the progress of glomerular sclerosis and tubulo-interstitial fibrosis in chronic kidney diseases. Chem Res Toxicol. Epub Jul Abstract Angelica sinensis is a Chinese medicinal herb for treating gynecological and gastrointestinal disorders, and also in conjunction with cancer chemotherapy. Am J Chin Med.

Angiogenic effects of the extracts from Chinese herbs: Angelica and Chuanxiong. Abstract Angelica and ChuanXiong are used to cure ischemic heart disease in China. Planta Med.

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